Image Analysis of Single DNA Molecules

The most accurate method for studying DNA replication is the labeling of newly synthesized DNA molecules with halogenated nucleosides followed by immunofluorescence and microscopy detection, known as DNA fiber labeling. The major difficulty of the method is the labor-intensive analysis, which requires measuring the lengths of a large number of labelled fragments. There are very few attempts to automate this analysis and software freely available to researchers is not available. In an attempt to solve the problem, the aim of my latest project was to develop an efficient software tool for the automated image analysis of single DNA molecules. The software is currently successfully used in the Institute of Molecular Biology of the Bulgarian Academy of Sciences.

Category: BIOLOGY Country: BULGARIA Year: 2021


Teodor Kirilov Kirilov